PARIS: Two genes controlling a tissue protein may have played a role in the key period when fish shed their fins and became limbed land-lovers, a study published by Nature on Thursday said.

Fossil evidence suggests that around 365 million years ago, fish, or fish-like creatures, emerged from shallow seas, moving onto land with the help of primitive, eight-fingered limbs, which later simplified to five digits under evolutionary pressure.

The newly-found genes control proteins called actinotrichia, whose tough, thin fibrils form a scaffold on which pectoral fins develop.

They were spotted by a team led by Marie-Andree Akimenko, from the University of Ottawa in Canada, as it was scanning development in the zebrafish, a highly-studied lab animal.

Neither of the genes are present in four-limbed vertebrates known as tetrapods, which became the basis for terrestrial animals, the researchers realised.

When the two genes were switched off in zebrafish embryos through genetic engineering, the fish developed only truncated fins, without bony rays.

The switchoff also unleashed a pattern of gene activity seen in research elsewhere, in the development of limbs and digits in terrestrial animals.

Further work is needed to confirm the theory, as it is unclear whether the fin genes were knocked out to help make the transition to land -- or whether they were eliminated after the transition, as they were no longer needed.

BDST: 1654 HRS. June 24, 2010
NJ/DC